diff --git a/.github/workflows/test_nixos_minimal.yml b/.github/workflows/test_nixos_minimal.yml
index 45e55ae14..4bb6f860a 100644
--- a/.github/workflows/test_nixos_minimal.yml
+++ b/.github/workflows/test_nixos_minimal.yml
@@ -17,6 +17,15 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       fail-fast: false
+      matrix:
+        test:
+          - name: hello
+            disk_size: 6144
+          - name: python
+            disk_size: 8192
+
+    name: Test ${{ matrix.test.name }}
+    timeout-minutes: 60
     steps:
       # In the subsequent tests, a relatively large disk (4GB) will be created to install AsterNixOS.
       # Standard GitHub runners may encounter "insufficient disk space" errors.
@@ -32,12 +41,11 @@ jobs:
           remove_haskell: true
           remove_tool_cache: true
       - uses: actions/checkout@v4
-      - name: Run the hello test on AsterNixOS
-        timeout-minutes: 30
+      - name: Run the ${{ matrix.test.name }} test on AsterNixOS
         uses: addnab/docker-run-action@v3
         with:
           image: asterinas/asterinas:0.17.0-20260114
           options: --privileged -v /dev:/dev -v ${{ github.workspace }}:/root/asterinas
           run: |
-            make nixos NIXOS_TEST_SUITE=hello NIXOS_DISK_SIZE_IN_MB=6144
-            make run_nixos NIXOS_TEST_SUITE=hello
\ No newline at end of file
+            make nixos NIXOS_TEST_SUITE=${{ matrix.test.name }} NIXOS_DISK_SIZE_IN_MB=${{ matrix.test.disk_size }}
+            make run_nixos NIXOS_TEST_SUITE=${{ matrix.test.name }}
diff --git a/test/nixos/Cargo.lock b/test/nixos/Cargo.lock
index 4e1a92452..167506724 100644
--- a/test/nixos/Cargo.lock
+++ b/test/nixos/Cargo.lock
@@ -266,6 +266,13 @@ dependencies = [
  "nixos-test-framework",
 ]
 
+[[package]]
+name = "test-python"
+version = "0.1.0"
+dependencies = [
+ "nixos-test-framework",
+]
+
 [[package]]
 name = "thiserror"
 version = "2.0.17"
diff --git a/test/nixos/tests/python/Cargo.toml b/test/nixos/tests/python/Cargo.toml
new file mode 100644
index 000000000..1d4c34a2e
--- /dev/null
+++ b/test/nixos/tests/python/Cargo.toml
@@ -0,0 +1,9 @@
+[package]
+name = "test-python"
+version.workspace = true
+repository.workspace = true
+license.workspace = true
+edition.workspace = true
+
+[dependencies]
+nixos-test-framework.workspace = true
\ No newline at end of file
diff --git a/test/nixos/tests/python/extra_config.nix b/test/nixos/tests/python/extra_config.nix
new file mode 100644
index 000000000..87628c6f5
--- /dev/null
+++ b/test/nixos/tests/python/extra_config.nix
@@ -0,0 +1,5 @@
+{ config, lib, pkgs, ... }:
+
+{
+  environment.systemPackages = with pkgs; [ python312 ];
+}
diff --git a/test/nixos/tests/python/src/main.rs b/test/nixos/tests/python/src/main.rs
new file mode 100644
index 000000000..7b62c330a
--- /dev/null
+++ b/test/nixos/tests/python/src/main.rs
@@ -0,0 +1,34 @@
+// SPDX-License-Identifier: MPL-2.0
+
+//! The test suite for Python regression tests on Asterinas NixOS.
+//!
+//! # Document maintenance
+//!
+//! An application's test suite and its "Verified Usage" section in Asterinas Book
+//! should always be kept in sync.
+//! So whenever you modify the test suite,
+//! review the documentation and see if should be updated accordingly.
+
+use nixos_test_framework::*;
+
+nixos_test_main!();
+
+#[nixos_test]
+fn python(nixos_shell: &mut Session) -> Result<(), Error> {
+    nixos_shell.run_cmd("curl https://www.python.org/ftp/python/3.12.12/Python-3.12.12.tar.xz -o Python-3.12.12.tar.xz")?;
+    nixos_shell.run_cmd("tar xf Python-3.12.12.tar.xz")?;
+    nixos_shell.run_cmd("export PYTHONPATH=$PWD/Python-3.12.12/Lib")?;
+
+    let testcases = std::fs::read_to_string("src/testcases.txt")?;
+    for line in testcases.lines() {
+        let line = line.trim();
+        if line.is_empty() || line.starts_with("#") {
+            continue;
+        }
+
+        let cmd = format!("python -m test -u all {}", line);
+        nixos_shell.run_cmd_and_expect(&cmd, "Result: SUCCESS")?;
+    }
+
+    Ok(())
+}
diff --git a/test/nixos/tests/python/src/testcases.txt b/test/nixos/tests/python/src/testcases.txt
new file mode 100644
index 000000000..fbc15dfdd
--- /dev/null
+++ b/test/nixos/tests/python/src/testcases.txt
@@ -0,0 +1,499 @@
+# This test list is generated based on the Python 3.12.12 source.
+# To regenerate this list, run the following commands:
+# curl https://www.python.org/ftp/python/3.12.12/Python-3.12.12.tar.xz -o Python-3.12.12.tar.xz
+# tar xf Python-3.12.12.tar.xz
+# export PYTHONPATH=$PWD/Python-3.12.12/Lib
+# python -m test --list-tests
+
+test.test_asyncio.test_base_events -i test_create_server_stream_bittype
+test.test_asyncio.test_buffered_proto
+test.test_asyncio.test_context
+test.test_asyncio.test_eager_task_factory
+test.test_asyncio.test_events -i python -m test -u all -v test.test_asyncio.test_events -i test_ssl_connect_accepted_socket -i test_server_close -i test_get_event_loop_new_process -i test_get_loop -i test_create_server* -i test_create_ssl*
+test.test_asyncio.test_futures
+test.test_asyncio.test_futures2
+test.test_asyncio.test_locks
+test.test_asyncio.test_pep492
+test.test_asyncio.test_proactor_events
+test.test_asyncio.test_protocols
+test.test_asyncio.test_queues
+test.test_asyncio.test_runners
+test.test_asyncio.test_selector_events
+test.test_asyncio.test_sendfile
+test.test_asyncio.test_server
+test.test_asyncio.test_sock_lowlevel -i test_cancel_sock_accept -i test_sock_client_fail -i test_recvfrom -i test_recvfrom_into -i test_sendto_blocking
+test.test_asyncio.test_ssl -i test_create_connection_memory_leak -i test_flush_before_shutdown -i test_create_connection_ssl* -i test_create_server_ssl* -i test_remote_shutdown_receives_trailing_data* -i test_start_tls* -i test_shutdown*
+test.test_asyncio.test_sslproto -i test_create_connection_memory_leak -i test_create_connection_ssl_failed_certificate -i test_start_tls*
+test.test_asyncio.test_staggered
+test.test_asyncio.test_streams -i test_open_connection_happy_eyeball_refcycles -i test_open_connection_no_loop_ssl -i test_open_unix_connection_no_loop_ssl
+test.test_asyncio.test_subprocess
+test.test_asyncio.test_taskgroups
+test.test_asyncio.test_tasks
+test.test_asyncio.test_threads
+test.test_asyncio.test_timeouts
+test.test_asyncio.test_transports
+test.test_asyncio.test_unix_events
+test.test_asyncio.test_waitfor
+test.test_asyncio.test_windows_events
+test.test_asyncio.test_windows_utils
+test.test_concurrent_futures.test_as_completed -i test_duplicate_futures -i test_future_times_out -i test_no_timeout -i tearDownModule
+test.test_concurrent_futures.test_deadlock -i ProcessPoolForkserverExecutorDeadlockTest
+test.test_concurrent_futures.test_future
+test.test_concurrent_futures.test_init -i ProcessPoolForkserverFailingInitializerTest -i ProcessPoolForkserverInitializerTest -i tearDownModule
+test.test_concurrent_futures.test_process_pool -i ProcessPoolForkserverProcessPoolExecutorTest
+test.test_concurrent_futures.test_shutdown -i ProcessPoolForkserverProcessPoolShutdownTest -i test_interpreter_shutdown -i test_cancel_futures_wait_false -i test_hang_gh83386
+test.test_concurrent_futures.test_thread_pool
+test.test_concurrent_futures.test_wait -i ProcessPoolForkserverWaitTest -i tearDownModule
+test.test_future_stmt.test_future
+test.test_future_stmt.test_future_flags
+test.test_future_stmt.test_future_multiple_features
+test.test_future_stmt.test_future_multiple_imports
+test.test_future_stmt.test_future_single_import
+test.test_gdb.test_backtrace
+test.test_gdb.test_cfunction
+test.test_gdb.test_cfunction_full
+test.test_gdb.test_misc
+test.test_gdb.test_pretty_print
+test.test_inspect.test_inspect -i test_details
+test.test_multiprocessing_fork.test_manager
+test.test_multiprocessing_fork.test_misc -i test_flags -i test_context -i test_set_get -i tearDownModule
+test.test_multiprocessing_fork.test_processes
+test.test_multiprocessing_fork.test_threads
+# test.test_multiprocessing_forkserver.test_manager
+test.test_multiprocessing_forkserver.test_misc -i test_closefd -i test_flags -i test_lock -i test_noforkbomb -i test_ignore* -i test_manager_initializer -i test_pool_initializer -i test_resource_tracker_reused -i test_empty -i test_context -i test_nested_startmethod -i test_preload_resources -i test_pool_in_process -i test_queue_in_process -i test_set_get -i TestSyncManagerTypes -i test_timeout -i test_neg_timeout -i test_wait* -i tearDownModule
+# test.test_multiprocessing_forkserver.test_processes
+test.test_multiprocessing_forkserver.test_threads -i test_rapid_restart
+test.test_multiprocessing_spawn.test_manager -i test_map_no_failfast
+test.test_multiprocessing_spawn.test_misc -i test_closefd -i test_flags -i test_empty -i test_context -i test_mixed_startmethod -i test_set_get -i test_wait_integer -i tearDownModule
+test.test_multiprocessing_spawn.test_processes -i WithProcessesTestBarrier -i test_notify* -i test_wait* -i test_event -i test_repr_lock -i test_repr_rlock -i test_map_no_failfast -i test_child_fd_inflation -i test_process -i test_get -i test_put -i test_task_done
+test.test_multiprocessing_spawn.test_threads
+test.test_pydoc.test_pydoc
+test___all__
+test__locale
+test__opcode
+test__osx_support
+test__xxinterpchannels
+test__xxsubinterpreters
+test_abc
+test_abstract_numbers
+test_aifc
+test_argparse
+test_array
+test_asdl_parser
+test_ast
+test_asyncgen
+test_atexit
+test_audioop
+test_audit
+test_augassign
+test_base64
+test_baseexception
+test_bdb
+test_bigaddrspace
+test_bigmem
+test_binascii
+test_binop
+test_bisect
+test_bool
+test_buffer
+test_bufio
+test_builtin -i test_divmod -i test_input_tty_non_ascii*
+test_bytes
+test_bz2
+test_c_locale_coercion
+test_calendar
+test_call
+test_capi
+# test_cext
+test_cgi
+test_cgitb
+test_charmapcodec
+test_class
+test_clinic
+test_cmath
+test_cmd
+test_cmd_line -i test_longexp -i test_relativedir_bug46421
+test_cmd_line_script -i test_directory* -i test_issue8202* -i test_module_in_* -i test_package* -i test_script* -i test_zipfile*
+test_code
+test_code_module
+test_codeccallbacks
+test_codecencodings_cn
+test_codecencodings_hk
+test_codecencodings_iso2022
+test_codecencodings_jp
+test_codecencodings_kr
+test_codecencodings_tw
+test_codecmaps_cn
+test_codecmaps_hk
+test_codecmaps_jp
+test_codecmaps_kr
+test_codecmaps_tw
+test_codecs
+test_codeop
+test_collections
+# test_colorsys
+test_compare
+test_compile
+test_compileall -i test_ddir_empty_multiple_workers -i test_ddir_multiple_workers -i test_workers
+test_compiler_assemble
+test_compiler_codegen
+test_complex
+test_configparser
+test_contains
+test_context
+test_contextlib
+test_contextlib_async
+test_copy
+test_copyreg
+test_coroutines
+# test_cppext
+test_cprofile
+test_crashers
+# test_crypt
+test_csv
+test_ctypes -i test_simple -i test_longdouble* -i test_find_library_with*
+test_curses
+test_dataclasses
+test_datetime
+test_dbm
+test_dbm_dumb
+test_dbm_gnu
+test_dbm_ndbm
+test_decimal
+test_decorators
+test_defaultdict
+test_deque
+test_descr -i test_floats
+test_descrtut
+test_devpoll
+test_dict
+test_dict_version
+test_dictcomps
+test_dictviews
+test_difflib
+test_dis
+test_doctest
+test_docxmlrpc
+test_dtrace
+test_dynamic
+test_dynamicclassattribute
+test_eintr
+test_email
+test_embed -i test_set_config
+test_ensurepip
+test_enum
+test_enumerate
+test_eof
+test_epoll
+test_errno
+test_except_star
+test_exception_group
+test_exception_hierarchy
+test_exception_variations
+test_exceptions
+test_extcall
+test_faulthandler
+test_fcntl -i test_fcntl_*
+test_file
+test_file_eintr
+test_filecmp
+test_fileinput -i test_buffer_sizes -i test_inplace* -i test_nextfile_oserror_deleting_backup -i test_pathlike_file_inplace -i test_readline_os*
+test_fileio -i testReadintoByteArray
+# test_fileutils
+test_finalization
+# test_float
+test_flufl
+test_fnmatch
+test_fork1
+test_format
+test_fractions -i testMixedIntegerDivision
+test_frame
+test_frozen
+test_fstring
+# test_ftplib
+test_funcattrs
+test_functools
+test_gc
+test_generator_stop
+test_generators
+test_genericalias
+test_genericclass
+test_genericpath
+test_genexps
+test_getopt
+test_getpass
+test_getpath
+test_gettext
+test_glob
+test_global
+test_grammar
+test_graphlib
+test_grp
+test_gzip
+test_hash
+test_hashlib
+test_heapq
+test_hmac
+test_html
+test_htmlparser
+test_http_cookiejar
+test_http_cookies
+test_httplib -i test_response_fileno -i HTTPSTest -i testHTTPConnectionSourceAddress
+test_httpservers -i test_undecodable_filename
+test_idle
+# test_imaplib
+test_imghdr
+test_import -i test_unencodable_filename
+test_importlib -i test_finder
+test_index
+test_int
+test_int_literal
+test_interpreters
+test_io -i test_large_file_ops -i test_interrupted_write_unbuffered
+test_ioctl
+test_ipaddress
+test_isinstance
+test_iter
+test_iterlen
+test_itertools
+test_json
+test_keyword
+test_keywordonlyarg
+test_kqueue
+# test_largefile
+test_launcher
+test_lib2to3
+test_linecache
+test_list
+test_listcomps
+test_lltrace
+test_locale
+test_logging -i test_output -i test_noserver -i test_max_bytes* -i test_namer_rotator_inheritance -i test_rollover*
+test_long
+test_longexp
+test_lzma
+test_mailbox -i test_nonempty* -i TestMaildir
+test_mailcap
+test_marshal
+test_math -i testRemainder
+test_math_property
+test_memoryio
+test_memoryview
+test_metaclass
+test_mimetypes
+test_minidom
+test_mmap
+test_module -i test_module_finalization_at_shutdown
+test_modulefinder
+test_monitoring
+test_msilib
+test_multibytecodec
+test_multiprocessing_main_handling -i ForkServerCmdLineTest
+test_named_expressions
+test_netrc
+test_nis
+test_nntplib -i test_starttls
+# test_ntpath
+test_numeric_tower
+test_opcache
+test_opcodes
+test_openpty
+test_operator
+test_optparse
+test_ordered_dict
+test_os -i test_getcwd_long_path -i test_listdir -i test_remove_nothing -i test_blocking -i test_dup -i test_dup2 -i test_dup2_negative_fd -i test_fchdir -i test_fchmod -i test_fchown -i test_fdatasync -i test_fdopen -i test_fpathconf -i test_fstat -i test_fstatvfs -i test_fsync -i test_ftruncate -i test_inheritable -i test_lseek -i test_read -i test_readv -i test_remove_partial -i test_replace -i test_tcgetpgrp -i test_tcsetpgrpt -i test_ttyname -i test_walk_bad_dir -i test_write -i test_writev
+test_ossaudiodev
+test_osx_env
+test_pathlib -i test_walk_bad_dir
+test_patma
+test_pdb -i test_basic_completion
+test_peepholer
+test_peg_generator
+test_pep646_syntax
+test_perf_profiler
+test_perfmaps
+test_pickle
+test_picklebuffer
+test_pickletools
+test_pipes
+test_pkg
+test_pkgutil
+test_platform
+test_plistlib
+test_poll
+test_popen
+# test_poplib
+test_positional_only_arg
+test_posix -i test_fchown -i test_lchown -i test_sched_rr_get_interval -i test_chown -i test_pipe2
+# test_posixpath
+test_pow
+test_pprint
+test_print
+test_profile
+test_property
+test_pstats
+test_pty
+test_pulldom
+test_pwd
+test_py_compile
+test_pyclbr
+test_pyexpat
+test_queue
+test_quopri
+test_raise
+test_random
+test_range
+test_re
+test_readline -i test_nonascii -i test_set_complete_delims
+test_regrtest -i ArgsTestCase -i test_finds_expected_number_of_tests -i test_module*
+test_repl
+test_reprlib
+test_resource
+test_richcmp
+test_rlcompleter
+test_robotparser
+test_runpy
+test_sax
+test_sched
+test_scope
+test_script_helper
+test_secrets
+test_select
+test_selectors -i test_register_file
+test_set
+test_setcomps
+test_shelve
+test_shlex
+test_shutil -i test_stty_match -i test_rmtree_errors* -i test_copyxattr_symlinks -i test_unpack_archive*
+# test_signal -i test_itimer_prof -i test_itimer_virtual -i test_wakeup_fd_during -i test_wakeup_fd_early
+test_site -i test_license_exists_at_url -i test_startup_imports
+test_slice
+test_smtplib
+# test_smtpnet
+test_sndhdr
+test_socket -i testDetach -i BasicUDPLITETest -i BasicUDPTest -i testRecvFromIntoEmptyBuffer -i testCreateConnectionClose -i testSourceAddress -i NetworkConnectionNoServer -i test_injected_authentication_failure -i testCmsgTrunc* -i testRecvmsgPeek -i testRecvmsgTrunc -i testSendmsgDontWait -i SendmsgUDPLITETest -i testUnencodableAddr -i UDPLITETimeoutTest -i test_tcp4 -i testCloseException -i testInterfaceNameIndex -i testSendtoErrors -i testSockName -i test_socket_fileno -i RecvmsgIntoUDPLITETest -i RecvmsgUDPLITETest
+test_socketserver
+test_sort
+test_source_encoding
+test_spwd
+test_sqlite3
+# test_ssl
+test_stable_abi_ctypes
+test_startfile
+test_stat
+test_statistics
+test_strftime
+test_string
+test_string_literals
+test_stringprep
+test_strptime
+test_strtod
+test_struct
+test_structseq
+test_subclassinit
+test_subprocess -i test_pipesize_default -i test_pipesizes
+test_sunau
+test_sundry
+test_super
+test_support -i test_rmtree
+test_symtable
+# test_syntax
+test_sys -i test_stdlib_dir
+test_sys_setprofile
+test_sys_settrace
+test_sysconfig
+test_syslog
+test_tabnanny -i test_command_usage
+test_tarfile -i test_extract_hardlink -i test_premature_end_of_archive -i test_fileobj_iter -i test_fileobj_readlines -i test_fileobj_seek -i test_sparse_file* -i test_deferred_directory_attributes_update -i TestExtractionFilters -i NoneInfoExtractTests_Data -i NoneInfoExtractTests_Tar
+test_tcl
+test_telnetlib
+test_tempfile
+test_termios -i test_tcdrain -i test_tcflow -i test_tcflush -i test_tcsendbreak -i test_tcflow_errors -i test_tcflush_errors
+test_textwrap
+test_thread
+test_threadedtempfile
+test_threading -i test_4_daemon_threads
+test_threading_local
+test_threadsignals
+test_time -i test_pthread_getcpuclockid -i test_clock_settime -i test_get_clock_info -i test_monotonic_settime -i test_process_time -i test_thread_time -i test_clock_getres
+test_timeit
+test_timeout
+test_tix
+test_tkinter
+test_tokenize
+test_tomllib
+test_tools -i test_freeze_simple_script
+test_trace
+test_traceback
+test_tracemalloc
+test_ttk
+test_ttk_textonly
+test_tty
+test_tuple
+test_turtle
+test_type_aliases
+test_type_annotations
+test_type_cache
+test_type_comments
+test_type_params
+test_typechecks
+test_types
+test_typing
+test_ucn
+test_unary
+test_unicode
+# test_unicode_file
+test_unicode_file_functions
+test_unicode_identifiers
+test_unicodedata
+test_unittest -i testSelectedTestNamesFunctionalTest
+test_univnewlines
+test_unpack
+test_unpack_ex
+test_unparse
+test_urllib
+test_urllib2
+test_urllib2_localnet -i test_https*
+test_urllib2net
+test_urllib_response
+test_urllibnet
+test_urlparse
+test_userdict
+test_userlist
+test_userstring
+test_utf8_mode
+test_utf8source
+test_uu
+test_uuid
+test_venv -i test_zippath_from_non_installed_posix -i test_with_pip -i test_prefixes -i test_sysconfig*
+test_wait3
+test_wait4
+test_warnings
+test_wave
+test_weakref -i test_atexit
+test_weakset
+test_webbrowser
+test_winapi
+test_winconsoleio
+test_winreg
+test_winsound
+test_with
+test_wmi
+test_wsgiref
+test_xdrlib
+test_xml_dom_minicompat
+test_xml_dom_xmlbuilder
+test_xml_etree
+test_xml_etree_c
+test_xmlrpc
+test_xmlrpc_net
+test_xxlimited
+test_xxtestfuzz
+test_yield_from
+test_zipapp
+test_zipfile
+# test_zipfile64
+test_zipimport
+test_zipimport_support
+test_zlib
+test_zoneinfo